植物染色体工程与表观遗传学团队
一、团队简介
太阳集团tyc5997“植物染色体工程与表观遗传学”团队。研究方向为植物表观遗传及分子细胞遗传学。团队先后承担20余项科研项目,发表SCI学术论文60余篇。申请发明专利十余项。
二、团队负责人简介
王凯,教授,博士生导师。教育部新世纪优秀人才。先后获 “闽江学者”特聘教授、“金山学者”特聘教授,福建省引进高层次人才等称号。曾任国家甘蔗工程技术研究中副主任、副经理等职务。曾获中华农业科技奖优秀创新团队奖(等同一等奖)、严家显最高奖教金、江苏省优秀百篇博士学位论文、江苏省遗传学会优秀论文评选特等奖等。主持各类科研项目20项,其中主持国家自然科学基金 6 项、教育部新世纪人才支持计划、国家重点研发计划项目,省自然科学基金等项目多项;指导研究生多人获得省级优秀毕业生和优秀学位论文。现已在Science, Nature Genetics, Genome Res、Plant Cell等期刊发表论文近70篇,参编英文著作1部,申请发明专利十余项。现任SCI杂志Frontiers in Plant Science,Chromosome Res编委,美国自然科学基金评委;担任国际棉花基因组创新研讨会会员,中国热带作物学会理事,福建省遗传学会理事。
主持课题:
2021 国家自然科学基金面上项目;
2019 国家重点研发计划子课题;
2018 国家自然科学基金面上项目
2017国家自然科学基金海外学者合作研究基金
2015 国家自然科学基金面上项目
2011 国家自然科学基金面上项目
2011教育部新世纪优秀人才支持计划项目
2008 国家自然科学基金青年科学基金
2008江苏省自然科学基金
2014闽江学者特聘教授
另外,主持云南省博士工作站及其他合作研究课题十余项。
三、团队其他成员
韩金磊,博士、校聘副教授。毕业于福建农林大学,从事植物表观遗传,基因表达调控等研究。参与完成多项科研项目。以第一作者在国际知名期刊Plant Cell 以及The Plant Journal发表学术论文。
四、团队主要研究方向
1)植物染色体工程遗传与育种应用;
2)作物重要性状决定基因的表达调控机制;
3)植物着丝粒结构与演化。
五、发表论文
2021年
Meng Z, Wang Q, Khurshid H, Raza G, Han J, Wang B, Wang K (2021) Chromosome Painting Provides Insights Into the Genome Structure and Evolution of Sugarcane. Front. Plant Sci, DOI 10.3389/fpls.2021.731664
Wang Y, Wang K (2021) Genome-Wide Identification of DNase I Hypersensitive Sites in Plants. Current protocols, 1:e148
2020年
Lin Q, Wang S, Dao Y, Wang J, Wang K (2020) The Arabidopsis thaliana trehalose-6-phosphate phosphatase gene AtTPPI enhances drought tolerance by regulating stomatal apertures. J Exp Bot, DOI 10.1093/jxb/eraa173
Hasterok R, Wang K, Jenkins G (2020) Progressive refinement of the karyotyping of Brachypodium genomes. New Phytol, DOI 10.1111/nph.16342
Meng Z, Han J, Lin Y, Zhao Y, Lin Q, Ma X, Wang J, Zhang M, Zhang L, Yang Q, Wang K (2020) Characterization of a Saccharum spontaneum with a basic chromosome number of x = 10 provides new insights on genome evolution in genus Saccharum. Theor Appl Genet 133:187-199
Huang Y, Chen H, Han J, Zhang Y, Ma S, Yu G, Wang Z, Wang K (2020) Species-specific abundant retrotransposons elucidate the genomic composition of modern sugarcane cultivars. Chromosoma 129:45-55
Zhang L, Chen F, Zhang X, Li Z, Zhao Y, Lohaus R, Chang X, Dong W, Ho SYW, Liu X, Song A, Chen J, Guo W, Wang Z, Zhuang Y, Wang H, Chen X, Hu J, Liu Y, Qin Y, Wang K, Dong S, Liu Y, Zhang S, Yu X, Wu Q, Wang L, Yan X, Jiao Y, Kong H, Zhou X, Yu C, Chen Y, Li F, Wang J, Chen W, Chen X, Jia Q, Zhang C, Jiang Y, Zhang W, Liu G, Fu J, Chen F, Ma H, Van de Peer Y, Tang H (2020) The water lily genome and the early evolution of flowering plants. Nature 577:79-84
Han J, Wang P, Wang Q, Lin Q, Chen Z, Yu G, Miao C, Dao Y, Wu R, Schnable JC, Tang H, Wang K (2020) Genome-wide characterization of DNase I-hypersensitive sites and cold response regulatory landscapes in grasses. Plant Cell 32:2457-2473
Wang Q, Yu G, Chen Z, Han J, Hu Y, Wang K (2020) Optimization of protoplast isolation, transformation and its application in sugarcane (Saccharum spontaneum L). The Crop Journal, 9:133-142
2019年
Wang S, Jin W, Wang K (2019) Centromere histone H3- and phospholipase-mediated haploid induction in plants. Plant Methods 15:42
Yu G, Wang J, Miao L, Xi M, Wang Q, Wang K (2019) Optimization of Mature Embryo-Based Tissue Culture and Agrobacterium-Mediated Transformation in Model Grass Brachypodium distachyon. Int J Mol Sci 20
Zou J, Mao L, Qiu J, Wang M, Jia L, Wu D, He Z, Chen M, Shen Y, Shen E, Huang Y, Li R, Hu D, Shi L, Wang K, Zhu Q, Ye C, Bancroft I, King GJ, Meng J, Fan L (2019) Genome-wide selection footprints and deleterious variations in young Asian allotetraploid rapeseed. Plant Biotechnol J, 17:1998-2010
Lin Q, Yang J, Wang Q, Zhu H, Chen Z, Dao Y, Wang K (2019) Overexpression of the trehalose-6-phosphate phosphatase family gene AtTPPF improves the drought tolerance of Arabidopsis thaliana. BMC Plant Biol 19:381
Li Z, Wang K (2019) Genome-Wide Identification of Regulatory DNA Elements in Crop Plants. In: Vaschetto LM (ed) Cereal Genomics: Methods and Protocols. Springer US, New York, NY, pp 85-99
Hu Y, Chen J, Fang L, Zhang Z, Ma W, Niu Y, Ju L, Deng J, Zhao T, Lian J, Baruch K, Fang D, Liu X, Ruan YL, Rahman MU, Han J, Wang K, Wang Q, Wu H, Mei G, Zang Y, Han Z, Xu C, Shen W, Yang D, Si Z, Dai F, Zou L, Huang F, Bai Y, Zhang Y, Brodt A, Ben-Hamo H, Zhu X, Zhou B, Guan X, Zhu S, Chen X, Zhang T (2019) Gossypium barbadense and Gossypium hirsutum genomes provide insights into the origin and evolution of allotetraploid cotton. Nat Genet 51:739-748
2018年
Zhang J, Zhang X, Tang H, Zhang Q, Hua X, Ma X, Zhu F, Jones T, Zhu X, Bowers J, Wai CM, Zheng C, Shi Y, Chen S, Xu X, Yue J, Nelson DR, Huang L, Li Z, Xu H, Zhou D, Wang Y, Hu W, Lin J, Deng Y, Pandey N, Mancini M, Zerpa D, Nguyen JK, Wang L, Yu L, Xin Y, Ge L, Arro J, Han JO, Chakrabarty S, Pushko M, Zhang W, Ma Y, Ma P, Lv M, Chen F, Zheng G, Xu J, Yang Z, Deng F, Chen X, Liao Z, Zhang X, Lin Z, Lin H, Yan H, Kuang Z, Zhong W, Liang P, Wang G, Yuan Y, Shi J, Hou J, Lin J, Jin J, Cao P, Shen Q, Jiang Q, Zhou P, Ma Y, Zhang X, Xu R, Liu J, Zhou Y, Jia H, Ma Q, Qi R, Zhang Z, Fang J, Fang H, Song J, Wang M, Dong G, Wang G, Chen Z, Ma T, Liu H, Dhungana SR, Huss SE, Yang X, Sharma A, Trujillo JH, Martinez MC, Hudson M, Riascos JJ, Schuler M, Chen LQ, Braun DM, Li L, Yu Q, Wang J, Wang K, Schatz MC, Heckerman D, Van Sluys MA, Souza GM, Moore PH, Sankoff D, VanBuren R, Paterson AH, Nagai C, Ming R (2018) Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L. Nat Genet 50:1565-1573
Sharma A, Song J, Lin Q, Singh R, Ramos N, Wang K, Zhang J, Ming R, Yu Q (2018) Comparative analysis of homologous sequences of Saccharum officinarum and Saccharum spontaneum reveals independent polyploidization events. Front Plant Sci 9:1414
Meng Z, Zhang Z, Yan T, Lin Q, Wang Y, Huang W, Huang Y, Li Z, Yu Q, Wang J, Wang K (2018) Comprehensively characterizing the cytological features of Saccharum spontaneum by the development of a complete set of chromosome-specific oligo probes. Front Plant Sci 9:1624
Liu S, Xue C, Fang Y, Chen G, Peng X, Zhou Y, Chen C, Liu G, Gu M, Wang K, Zhang W, Wu Y, Gong Z (2018) Global Involvement of Lysine Crotonylation in Protein Modification and Transcription Regulation in Rice. Mol Cell Proteomics 17:1922-1936
Li Y, Zuo S, Zhang Z, Li Z, Han J, Chu Z, Hasterok R, Wang K (2018) Centromeric DNA characterization in the model grass Brachypodium distachyon provides insights on the evolution of the genus. Plant J 93:1088-1101
Han J, Zhang Z, Wang K (2018) 3C and 3C-based techniques: the powerful tools for spatial genome organization deciphering. Mol Cytogenet 11:21
Dong G, Shen J, Zhang Q, Wang J, Yu Q, Ming R, Wang K, Zhang J (2018) Development and applications of chromosome-specific cytogenetic BAC-FISH Probes in S. spontaneum. Front Plant Sci 9:218
2017年:
Pan X, Fang Y, Yang X, Zheng D, Chen L, Wang L, Xiao J, Wang XE, Wang K, Cheng Z, Yu H, Zhang W (2017) Chromatin states responsible for the regulation of differentially expressed genes under (60)Co~gamma ray radiation in rice. BMC Genomics 18:778
Zhang W, Zuo S, Li Z, Meng Z, Han J, Song J, Pan YB, Wang K (2017) Isolation and characterization of centromeric repetitive DNA sequences in Saccharum spontaneum. Sci Rep 7:41659
韩金磊, 吴为人,王凯 (2016). 染色体构象及其研究方法—3C技术. 分子植物育种(12): 3501-3510.
2016:
Han J, Masonbrink RE, Shan W, Song F, Zhang J, Yu W, Wang K, Wu Y, Tang H, Wendel JF, Wang K (2016) Rapid proliferation and nucleolar organizer targeting centromeric retrotransposons in cotton. Plant J 88:992-1005
Wang K, Yu W (2016) Chromosome Preparation in Rice (Oryza sativa). In: Gary Stacey, Birchler J, Ecker J, Martin C, Stitt M, Zhou J-M (eds) Current Protocols in Plant Biology. John Wiley & Sons, Inc., Hoboken, New Jersey ,USA, pp 67-77
Wang K, Yu W (2016) In Situ Hybridization in Rice (Oryza sativa). In: Gary Stacey, Birchler J, Ecker J, Martin C, Stitt M, Zhou J-M (eds) Current Protocols in Plant Biology. John Wiley & Sons, Inc., Hoboken, New Jersey ,USA, pp 89-106
Shan W, Jiang Y, Han J, Wang K (2016) Comprehensive cytological characterization of the Gossypium hirsutum genome based on the development of a set of chromosome cytological markers. Crop Journal 4:256-265
--2015:
Han J, Zhou B, Shan W, Yu L, Wu W, Wang K (2015) A and D genomes spatial separation at somatic metaphase in tetraploid cotton: evidence for genomic disposition in a polyploid plant. Plant J 84:1167-1177
Gent JI, Wang K, Jiang J, Dawe RK (2015) Stable patterns of CENH3 occupancy through maize lineages containing genetically similar centromeres. Genetics 200:1105-1116
Wang K, Wu Y, Zhang W, Dawe RK, Jiang J (2014) Maize centromeres expand and adopt a uniform size in the genetic background of oat. Genome Res 24:107-116
Zhang H, Koblizkova A, Wang K, Gong Z, Oliveira L, Torres GA, Wu Y, Zhang W, Novak P, Buell CR, Macas J, Jiang J (2014) Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres. Plant Cell 26:1436-1447
Cook DE, Bayless AM, Wang K, Guo X, Song Q, Jiang J, Bent AF (2014) Distinct copy number, coding sequence, and locus methylation patterns underlie Rhg1-Mediated soybean resistance to soybean cyst nematode. Plant Physiol 165:630-647
Chen Y, Wang Y, Wang K, Zhu X, Guo W, Zhang T, Zhou B (2014) Construction of a complete set of alien chromosome addition lines from Gossypium australe in Gossypium hirsutum: morphological, cytological, and genotypic characterization. Theor Appl Genet 127:1105-1121
Wang K, Zhang W, Jiang Y, Zhang T (2013) Systematic application of DNA fiber-FISH technique in cotton. PLoS One, 8:e75674
Cook DE, Lee TG, Guo X, Melito S, Wang K, Bayless AM, Wang J, Hughes TJ, Willis DK, Clemente TE, Diers BW, Jiang J, Hudson ME, Bent AF (2012) Copy number variation of multiple genes at Rhg1 mediates nematode resistance in soybean. Science 338:1206-1209
Wang K, Zhang W, Cao Y, Zhang Z, Zheng D, Zhou B, Guo W, Zhang T (2012) Localization of high level of sequence conservation and divergence regions in cotton. Theor Appl Genet 124:1173-1182
Gong Z, Wu Y, Koblizkova A, Torres GA, Wang K, Iovene M, Neumann P, Zhang W, Novak P, Buell CR, Macas J, Jiang J (2012) Repeatless and repeat-based centromeres in potato: Implications for centromere evolution. Plant Cell 24:3559-3574
Zhang H, Phan BH, Wang K, Artelt BJ, Jiang J, Parrott WA, Dawe RK (2012) Stable integration of an engineered megabase repeat array into the maize genome. Plant J 70:357-365
Wang K, Guo W, Yang Z, Hu Y, Zhang W, Zhou B, Stelly D, Chen Z, Zhang T (2010) Structure and size variations between 12A and 12D homoeologous chromosomes based on high-resolution cytogenetic map in allotetraploid cotton. Chromosoma 119:255-266
Wang K, Yang Z, Shu C, Hu J, Lin Q, Zhang W, Guo W, Zhang T (2009) Higher axial-resolution and sensitivity pachytene fluorescence in situ hybridization protocol in tetraploid cotton. Chromosome Res 17:1041-1050
Wang K, Guan B, Guo W, Zhou B, Hu Y, Zhu Y, Zhang T (2008) Completely distinguishing individual A-genome chromosomes and their karyotyping analysis by multiple bacterial artificial chromosome-fluorescence in situ hybridization. Genetics 178:1117-1122
Guan B, Wang K, Zhou BL, Guo WZ, Zhang TZ (2008) Establishment of a multi-color genomic in situ hybridization technique to simultaneously discriminate the three interspecific hybrid genomes in gossypium. J Integr Plant Biol 50:345-351
Wang K, Guo W, Zhang T (2007) Detection and mapping of homologous and homoeologous segments in homoeologous groups of allotetraploid cotton by BAC-FISH. BMC Genomics 8:178
Wang K, Guo W, Zhang T (2007) Development of one set of chromosome-specific microsatellite-containing BACs and their physical mapping in Gossypium hirsutum L. Theor Appl Genet 115:675-682
Guo W, Cai C, Wang C, Han Z, Song X, Wang K, Niu X, Wang C, Lu K, Shi B, Zhang T (2007) A microsatellite-based, gene-rich linkage map reveals genome structure, function and evolution in Gossypium. Genetics 176:527-541
Wang K, Song X, Han Z, Guo W, Yu JZ, Sun J, Pan J, Kohel RJ, Zhang T (2006) Complete assignment of the chromosomes of Gossypium hirsutum L. by translocation and fluorescence in situ hybridization mapping. Theor Appl Genet 113:73-80
Song X, Wang K, Guo W, Zhang J, Zhang T (2005) A comparison of genetic maps constructed from haploid and BC1 mapping populations from the same crossing between Gossypium hirsutum L. and Gossypium barbadense L. Genome 48:378-390
六、招聘启事
因研究工作需要,本团队诚招遗传学、生物信息学等相关专业出站博士后或应届博士毕业生。
应聘资格:
1.热爱科研,工作积极主动,态度严谨,责任心强,具备良好的团队合作精神。
2.具备独立开展科研的能力;以第一作者身份发表过SCI论文至少3篇(其中至少1篇中科院二区)。
相关待遇:详见太阳成集团tyc151cc人事处网站https://rsc.ntu.edu.cn/xgdy/list.htm
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联系人:王老师、韩老师